Ontology highlight
ABSTRACT:
SUBMITTER: Fimiani C
PROVIDER: S-EPMC4652751 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Fimiani Cristina C Goina Elisa E Mallamaci Antonello A
Nucleic acids research 20150707 16
To promote expression of endogenous genes ad libitum, we developed a novel, programmable transcription factor prototype. Kept together via an MS2 coat protein/RNA interface, it includes a fixed, polypeptidic transactivating domain and a variable RNA domain that recognizes the desired gene. Thanks to this device, we specifically upregulated five genes, in cell lines and primary cultures of murine pallial precursors. Gene upregulation was small, however sufficient to robustly inhibit neuronal diff ...[more]