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Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure Design.

ABSTRACT: A bimolecular ribozyme consisting of a core ribozyme (?P5 RNA) and an activator module (P5abc RNA) has been used as a platform to design assembled RNA nanostructures. The tight and specific assembly between the P5abc and ?P5 modules depends on two sets of intermodule interactions. The interface between P5abc and ?P5 must be controlled when designing RNA nanostructures. To expand the repertoire of molecular recognition in the P5abc/?P5 interface, we modified the interface by replacing the parent tertiary interactions in the interface with artificial interactions. The engineered P5abc/?P5 interfaces were characterized biochemically to identify those suitable for nanostructure design. The new interfaces were used to construct 2D-square and 1D-array RNA nanostructures.

SUBMITTER: Rahman MM

PROVIDER: S-EPMC5745442 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure Design.

Rahman Md Motiar MM Matsumura Shigeyoshi S Ikawa Yoshiya Y

Biology 20171030 4

A bimolecular ribozyme consisting of a core ribozyme (ΔP5 RNA) and an activator module (P5abc RNA) has been used as a platform to design assembled RNA nanostructures. The tight and specific assembly between the P5abc and ΔP5 modules depends on two sets of intermodule interactions. The interface between P5abc and ΔP5 must be controlled when designing RNA nanostructures. To expand the repertoire of molecular recognition in the P5abc/ΔP5 interface, we modified the interface by replacing the parent ...[more]

PMID: 29084145

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