Ontology highlight
ABSTRACT:
SUBMITTER: Nunez JK
PROVIDER: S-EPMC4662619 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Nuñez James K JK Harrington Lucas B LB Kranzusch Philip J PJ Engelman Alan N AN Doudna Jennifer A JA
Nature 20151021 7579
Bacteria and archaea generate adaptive immunity against phages and plasmids by integrating foreign DNA of specific 30-40-base-pair lengths into clustered regularly interspaced short palindromic repeat (CRISPR) loci as spacer segments. The universally conserved Cas1-Cas2 integrase complex catalyses spacer acquisition using a direct nucleophilic integration mechanism similar to retroviral integrases and transposases. How the Cas1-Cas2 complex selects foreign DNA substrates for integration remains ...[more]