Ontology highlight
ABSTRACT:
SUBMITTER: Szabat M
PROVIDER: S-EPMC4666348 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
PloS one 20151118 11
This study investigated the influence of the nature of oligonucleotides on the abilities to form antiparallel and parallel duplexes. Base pairing of homopurine DNA, 2'-O-MeRNA and RNA oligonucleotides with respective homopyrimidine DNA, 2'-O-MeRNA and RNA as well as chimeric oligonucleotides containing LNA resulted in the formation of 18 various duplexes. UV melting, circular dichroism and fluorescence studies revealed the influence of nucleotide composition on duplex structure and thermal stabi ...[more]