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Native sulfur/chlorine SAD phasing for serial femtosecond crystallography.

ABSTRACT: Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77?Å is successfully demonstrated. This sulfur SAD method can be applied to a wide range of proteins, which will improve the determination of native crystal structures.

SUBMITTER: Nakane T

PROVIDER: S-EPMC4667287 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Native sulfur/chlorine SAD phasing for serial femtosecond crystallography.

Nakane Takanori T Song Changyong C Suzuki Mamoru M Nango Eriko E Kobayashi Jun J Masuda Tetsuya T Inoue Shigeyuki S Mizohata Eiichi E Nakatsu Toru T Tanaka Tomoyuki T Tanaka Rie R Shimamura Tatsuro T Tono Kensuke K Joti Yasumasa Y Kameshima Takashi T Hatsui Takaki T Yabashi Makina M Nureki Osamu O Iwata So S Sugahara Michihiro M

Acta crystallographica. Section D, Biological crystallography 20151127 Pt 12

Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77 Å is successfully demonstrated. This sulfur SAD method can be applied to a wide range of proteins, which will improve the determination of native cr ...[more]

PMID: 26627659

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