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A mitochondria-targeted ratiometric two-photon fluorescent probe for biological zinc ions detection.

ABSTRACT: A mitochondria-targeted ratiometric two-photon fluorescent probe (Mito-MPVQ) for biological zinc ions detection was developed based on quinolone platform. Mito-MPVQ showed large red shifts (68 nm) and selective ratiometric signal upon Zn(2+) binding. The ratio of emission intensity (I488 nm/I420 nm) increases dramatically from 0.45 to 3.79 (ca. 8-fold). NMR titration and theoretical calculation confirmed the binding of Mito-MPVQ and Zn(2+). Mito-MPVQ also exhibited large two-photon absorption cross sections (150 GM) at nearly 720 nm and insensitivity to pH within the biologically relevant pH range. Cell imaging indicated that Mito-MPVQ could efficiently located in mitochondria and monitor mitochondrial Zn(2+) under two-photon excitation with low cytotoxicity.

SUBMITTER: Ning P

PROVIDER: S-EPMC4673014 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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A mitochondria-targeted ratiometric two-photon fluorescent probe for biological zinc ions detection.

Ning Peng P Jiang Jiacheng J Li Longchun L Wang Shuxin S Yu Haizhu H Feng Yan Y Zhu Manzhou M Zhang Buchang B Yin Hang H Guo Qingxiang Q Meng Xiangming X

Biosensors & bioelectronics 20151026

A mitochondria-targeted ratiometric two-photon fluorescent probe (Mito-MPVQ) for biological zinc ions detection was developed based on quinolone platform. Mito-MPVQ showed large red shifts (68 nm) and selective ratiometric signal upon Zn(2+) binding. The ratio of emission intensity (I488 nm/I420 nm) increases dramatically from 0.45 to 3.79 (ca. 8-fold). NMR titration and theoretical calculation confirmed the binding of Mito-MPVQ and Zn(2+). Mito-MPVQ also exhibited large two-photon absorption cr ...[more]

PMID: 26528806

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