Ontology highlight
ABSTRACT:
SUBMITTER: Tsai YH
PROVIDER: S-EPMC4673907 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Tsai Yu-Hsuan YH Essig Sebastian S James John R JR Lang Kathrin K Chin Jason W JW
Nature chemistry 20150518 7
The rapid and selective regulation of a target protein within living cells that contain closely related family members is an outstanding challenge. Here we introduce genetically directed bioorthogonal ligand tethering (BOLT) and demonstrate selective inhibition (iBOLT) of protein function. In iBOLT, inhibitor-conjugate/target protein pairs are created where the target protein contains a genetically encoded unnatural amino acid with bioorthogonal reactivity and the inhibitor conjugate contains a ...[more]