Dataset Information

A Novel Approach to High-Quality Postmortem Tissue Procurement: The GTEx Project.

ABSTRACT: The Genotype-Tissue Expression (GTEx) project, sponsored by the NIH Common Fund, was established to study the correlation between human genetic variation and tissue-specific gene expression in non-diseased individuals. A significant challenge was the collection of high-quality biospecimens for extensive genomic analyses. Here we describe how a successful infrastructure for biospecimen procurement was developed and implemented by multiple research partners to support the prospective collection, annotation, and distribution of blood, tissues, and cell lines for the GTEx project. Other research projects can follow this model and form beneficial partnerships with rapid autopsy and organ procurement organizations to collect high quality biospecimens and associated clinical data for genomic studies. Biospecimens, clinical and genomic data, and Standard Operating Procedures guiding biospecimen collection for the GTEx project are available to the research community.

SUBMITTER: Carithers LJ

PROVIDER: S-EPMC4675181 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Publications

A Novel Approach to High-Quality Postmortem Tissue Procurement: The GTEx Project.

Carithers Latarsha J LJ Ardlie Kristin K Barcus Mary M Branton Philip A PA Britton Angela A Buia Stephen A SA Compton Carolyn C CC DeLuca David S DS Peter-Demchok Joanne J Gelfand Ellen T ET Guan Ping P Korzeniewski Greg E GE Lockhart Nicole C NC Rabiner Chana A CA Rao Abhi K AK Robinson Karna L KL Roche Nancy V NV Sawyer Sherilyn J SJ Segrè Ayellet V AV Shive Charles E CE Smith Anna M AM Sobin Leslie H LH Undale Anita H AH Valentino Kimberly M KM Vaught Jim J Young Taylor R TR Moore Helen M HM

Biopreservation and biobanking 20151001 5

The Genotype-Tissue Expression (GTEx) project, sponsored by the NIH Common Fund, was established to study the correlation between human genetic variation and tissue-specific gene expression in non-diseased individuals. A significant challenge was the collection of high-quality biospecimens for extensive genomic analyses. Here we describe how a successful infrastructure for biospecimen procurement was developed and implemented by multiple research partners to support the prospective collection, a ...[more]

PMID: 26484571

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Project description:High-quality, well-annotated, healthy tissue specimens are crucial to the success of basic and translational research, but often difficult to procure. Postmortem (PM) tissue collections provide the opportunity to collect these healthy biospecimens. PM procurement programs led by biobanks can further contribute by providing researchers with rare biospecimens collected with short postmortem intervals (PMI) in controlled environments. To support biomedical and translational research, the Cornell Veterinary Biobank (CVB), an ISO 20387 accredited core resource at the Cornell University College of Veterinary Medicine, has performed PM tissue collections from research and privately owned animals since 2013. The CVB PM collection team, consisting of a board-certified veterinary pathologist, a licensed veterinary technician collection specialist, and a data capture specialist, performs rapid tissue collections during controlled warm necropsies, with an accepted PMI of ≤2 hours and a target PMI of ≤1 hour. A retrospective analysis of PM collections between 2013 and 2020 was completed, consisting of 4077 aliquots of 1582 biospecimens from 69 donors (48 canine, 16 feline, and 5 equine). An average of 22.93 biospecimens per donor were collected (range: 1-49). The average PMI for standard collections was 43.48 ± 2.30 minutes, starting on average 20.81 ± 1.61 minutes after time of death. Thus far, the CVB has a favorable utilization rate, with 414 aliquots (10.15%) from 350 specimens (20.12%) and 45 animals (65.22%) distributed to researchers. The success of the CVB PM tissue biobanking program, collecting high-quality biospecimens with short PMIs, was due to support from veterinary pathologists, the competence of CVB personnel, and the continuous evolution of methods within a quality management system. Improvement of PM tissue collection programs in biobanks, with standardized practices for all processes and specialized personnel, can enhance the quality and increase utilization of its biospecimens and associated data.

Dataset Information

A Novel Approach to High-Quality Postmortem Tissue Procurement: The GTEx Project.

Publications

A Novel Approach to High-Quality Postmortem Tissue Procurement: The GTEx Project.

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