Compact Wireless Microscope for In-Situ Time Course Study of Large Scale Cell Dynamics within an Incubator.

ABSTRACT: Imaging of live cells in a region of interest is essential to life science research. Unlike the traditional way that mounts CO2 incubator onto a bulky microscope for observation, here we propose a wireless microscope (termed w-SCOPE) that is based on the "microscope-in-incubator" concept and can be easily housed into a standard CO2 incubator for prolonged on-site observation of the cells. The w-SCOPE is capable of tunable magnification, remote control and wireless image transmission. At the same time, it is compact, measuring only ~10?cm in each dimension, and cost-effective. With the enhancement of compressive sensing computation, the acquired images can achieve a wide field of view (FOV) of ~113?mm(2) as well as a cellular resolution of ~3??m, which enables various forms of follow-up image-based cell analysis. We performed 12?hours time-lapse study on paclitaxel-treated MCF-7 and HEK293T cell lines using w-SCOPE. The analytic results, such as the calculated viability and therapeutic window, from our device were validated by standard cell detection assays and imaging-based cytometer. In addition to those end-point detection methods, w-SCOPE further uncovered the time course of the cell's response to the drug treatment over the whole period of drug exposure.

SUBMITTER: Jin D 

PROVIDER: S-EPMC4683435 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature