DNA Brushing Shoulders: Targeted Looping and Scanning of Large DNA Strands.
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ABSTRACT:
SUBMITTER: Azad Z
PROVIDER: S-EPMC4684187 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Publications
DNA Brushing Shoulders: Targeted Looping and Scanning of Large DNA Strands.
Azad Zubair Z Roushan Maedeh M Riehn Robert R
Nano letters 20150713 8
We present a nanofluidic device for targeted manipulations in the quarternary structure of single DNA molecules. We demonstrate the folding and unfolding of hairpin-shaped regions, similar to chromatin loops. These loops are stable for minutes at nanochannel junctions. We demonstrate continuous scanning of two DNA segments that occupy a common nanovolume. We present a model governing the stability of loop folds and discuss how the system achieves specific DNA configurations without operator inte ...[more]
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