Ontology highlight
ABSTRACT:
SUBMITTER: Amore A
PROVIDER: S-EPMC4686458 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Amore Antonella A Iadonisi Alfonso A Vincent Florence F Faraco Vincenza V
AMB Express 20151221 1
In this study, the recombinant α-L-arabinofuranosidase from the fungus Pleurotus ostreatus (rPoAbf) was subjected to site-directed mutagenesis in order to identify the catalytic nucleophile residue. Based on bioinformatics and homology modelling analyses, E449 was revealed to be the potential nucleophilic residue. Thus, the mutant E449G of PoAbf was recombinantly expressed in Pichia pastoris and its recombinant expression level and reactivity were investigated in comparison to the wild-type. The ...[more]