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Genome editing. The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations.

ABSTRACT: An organism with a single recessive loss-of-function allele will typically have a wild-type phenotype, whereas individuals homozygous for two copies of the allele will display a mutant phenotype. We have developed a method called the mutagenic chain reaction (MCR), which is based on the CRISPR/Cas9 genome-editing system for generating autocatalytic mutations, to produce homozygous loss-of-function mutations. In Drosophila, we found that MCR mutations efficiently spread from their chromosome of origin to the homologous chromosome, thereby converting heterozygous mutations to homozygosity in the vast majority of somatic and germline cells. MCR technology should have broad applications in diverse organisms.

SUBMITTER: Gantz VM

PROVIDER: S-EPMC4687737 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Genome editing. The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations.

Gantz Valentino M VM Bier Ethan E

Science (New York, N.Y.) 20150319 6233

An organism with a single recessive loss-of-function allele will typically have a wild-type phenotype, whereas individuals homozygous for two copies of the allele will display a mutant phenotype. We have developed a method called the mutagenic chain reaction (MCR), which is based on the CRISPR/Cas9 genome-editing system for generating autocatalytic mutations, to produce homozygous loss-of-function mutations. In Drosophila, we found that MCR mutations efficiently spread from their chromosome of o ...[more]

PMID: 25908821

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