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Modulation of IL-27 in adipocytes during inflammatory stress.

ABSTRACT: While it is well established that adipose tissue-derived inflammation plays an important role in the pathogenic mechanisms linking obesity with metabolic dysfunction, the inflammatory mediators involved have not been fully elucidated. Here, we explored IL-12 family cytokines with a focus on IL-27 during obesity-induced inflammation in mice and cultured adipocytes (ADs) following exposure to inflammatory stimuli.Relative mRNA abundance of IL-12 cytokines was assessed by reverse transcription polymerase chain reaction (RT-PCR) in genetically obese B6-ob/ob mice as well as C57BL/6J mice fed a high-fat diet and in ADs following exposure to inflammatory stimuli. Protein secretion of cytokines into culture media was assessed by ELISA, and the biological outcome of IL-27 stimulation was assessed by RT-PCR and immunoblotting.Heterodimeric subunits constituting IL-27 were significantly induced in obese mice. While all IL-12 genes were markedly induced by inflammatory stress in cultured ADs, IL-27 protein was the only cytokine secreted into culture media in response to inflammatory stress. Cultured ADs also responded to IL-27 stimulation with divergent outcomes that were dependent on the inflammatory milieu of target cells.These findings support the premise of autocrine/paracrine mechanisms involving IL-27 in ADs under conditions of inflammatory stress that may link obesity with inflammatory diseases.

SUBMITTER: Nam H

PROVIDER: S-EPMC4688214 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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Modulation of IL-27 in adipocytes during inflammatory stress.

Nam Heesun H Ferguson Bradley S BS Stephens Jacqueline M JM Morrison Ron F RF

Obesity (Silver Spring, Md.) 20151206 1

<h4>Objective</h4>While it is well established that adipose tissue-derived inflammation plays an important role in the pathogenic mechanisms linking obesity with metabolic dysfunction, the inflammatory mediators involved have not been fully elucidated. Here, we explored IL-12 family cytokines with a focus on IL-27 during obesity-induced inflammation in mice and cultured adipocytes (ADs) following exposure to inflammatory stimuli.<h4>Methods</h4>Relative mRNA abundance of IL-12 cytokines was asse ...[more]

PMID: 26638127

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Modulation of IL-27 in adipocytes during inflammatory stress.

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Modulation of IL-27 in adipocytes during inflammatory stress.

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