Ontology highlight
ABSTRACT:
SUBMITTER: Martinez-Bonet M
PROVIDER: S-EPMC4689412 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Martínez-Bonet Marta M Palladino Claudia C Briz Veronica V Rudolph Jochen M JM Fackler Oliver T OT Relloso Miguel M Muñoz-Fernandez Maria Angeles MA Madrid Ricardo R
PloS one 20151223 12
To find out new determinants required for Nef activity we performed a functional alanine scanning analysis along a discrete but highly conserved region at the core of HIV-1 Nef. We identified the GPG-motif, located at the 121-137 region of HIV-1 NL4.3 Nef, as a novel protein signature strictly required for the p56Lck dependent Nef-induced CD4-downregulation in T-cells. Since the Nef-GPG motif was dispensable for CD4-downregulation in HeLa-CD4 cells, Nef/AP-1 interaction and Nef-dependent effects ...[more]