Unknown

Dataset Information

0

Autoantibodies targeting glomerular annexin A2 identify patients with proliferative lupus nephritis.


ABSTRACT: Patients with systemic lupus erythematosus (SLE) frequently develop lupus nephritis (LN), a complication frequently leading to end stage kidney disease. Immune complex deposition in the glomerulus is central to the development of LN. Using a targeted proteomic approach, we tested the hypothesis that autoantibodies targeting glomerular antigens contribute to the development of LN.Human podocyte and glomerular proteins were separated by SDS-PAGE and immunoblotted with sera from SLE patients with and without LN. The regions of those gels corresponding to reactive bands observed with sera from LN patients were analyzed using LC-MS/MS.LN reactive bands were seen at approximately 50 kDa in podocyte extracts and between 36 and 50 kDa in glomerular extracts. Those bands were analyzed by LC-MS/MS and 102 overlapping proteins were identified. Bioinformatic analysis determined that 36 of those proteins were membrane associated, including a protein previously suggested to contribute to glomerulonephritis and LN, annexin A2. By ELISA, patients with proliferative LN demonstrated significantly increased antibodies against annexin A2.Proteomic approaches identified multiple candidate antigens for autoantibodies in patients with LN. Serum antibodies against annexin A2 were significantly elevated in subjects with proliferative LN, validating those antibodies as potential biomarkers.

SUBMITTER: Caster DJ 

PROVIDER: S-EPMC4690797 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Autoantibodies targeting glomerular annexin A2 identify patients with proliferative lupus nephritis.

Caster Dawn J DJ   Korte Erik A EA   Merchant Michael L ML   Klein Jon B JB   Wilkey Daniel W DW   Rovin Brad H BH   Birmingham Dan J DJ   Harley John B JB   Cobb Beth L BL   Namjou Bahram B   McLeish Kenneth R KR   Powell David W DW  

Proteomics. Clinical applications 20150612 11-12


<h4>Purpose</h4>Patients with systemic lupus erythematosus (SLE) frequently develop lupus nephritis (LN), a complication frequently leading to end stage kidney disease. Immune complex deposition in the glomerulus is central to the development of LN. Using a targeted proteomic approach, we tested the hypothesis that autoantibodies targeting glomerular antigens contribute to the development of LN.<h4>Experimental design</h4>Human podocyte and glomerular proteins were separated by SDS-PAGE and immu  ...[more]

Similar Datasets

| S-EPMC6176168 | biostudies-literature
| S-EPMC11001354 | biostudies-literature
| S-EPMC4214525 | biostudies-literature
| S-EPMC4493823 | biostudies-literature
| S-EPMC7566710 | biostudies-literature
| S-EPMC5025237 | biostudies-literature
2015-06-10 | GSE69662 | GEO
| S-EPMC5837387 | biostudies-literature
| S-EPMC8205752 | biostudies-literature
| S-EPMC3334650 | biostudies-literature