Unknown

Dataset Information

0

CdiA promotes receptor-independent intercellular adhesion.


ABSTRACT: CdiB/CdiA proteins mediate inter-bacterial competition in a process termed contact-dependent growth inhibition (CDI). Filamentous CdiA exoproteins extend from CDI(+) cells and bind specific receptors to deliver toxins into susceptible target bacteria. CDI has also been implicated in auto-aggregation and biofilm formation in several species, but the contribution of CdiA-receptor interactions to these multi-cellular behaviors has not been examined. Using Escherichia coli isolate EC93 as a model, we show that cdiA and bamA receptor mutants are defective in biofilm formation, suggesting a prominent role for CdiA-BamA mediated cell-cell adhesion. However, CdiA also promotes auto-aggregation in a BamA-independent manner, indicating that the exoprotein possesses an additional adhesin activity. Cells must express CdiA in order to participate in BamA-independent aggregates, suggesting that adhesion could be mediated by homotypic CdiA-CdiA interactions. The BamA-dependent and BamA-independent interaction domains map to distinct regions within the CdiA filament. Thus, CdiA orchestrates a collective behavior that is independent of its growth-inhibition activity. This adhesion should enable 'greenbeard' discrimination, in which genetically unrelated individuals cooperate with one another based on a single shared trait. This kind-selective social behavior could provide immediate fitness benefits to bacteria that acquire the systems through horizontal gene transfer.

SUBMITTER: Ruhe ZC 

PROVIDER: S-EPMC4694591 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

CdiA promotes receptor-independent intercellular adhesion.

Ruhe Zachary C ZC   Townsley Loni L   Wallace Adam B AB   King Andrew A   Van der Woude Marjan W MW   Low David A DA   Yildiz Fitnat H FH   Hayes Christopher S CS  

Molecular microbiology 20150730 1


CdiB/CdiA proteins mediate inter-bacterial competition in a process termed contact-dependent growth inhibition (CDI). Filamentous CdiA exoproteins extend from CDI(+) cells and bind specific receptors to deliver toxins into susceptible target bacteria. CDI has also been implicated in auto-aggregation and biofilm formation in several species, but the contribution of CdiA-receptor interactions to these multi-cellular behaviors has not been examined. Using Escherichia coli isolate EC93 as a model, w  ...[more]

Similar Datasets

| S-EPMC6170782 | biostudies-literature
| S-EPMC1276707 | biostudies-literature
| S-EPMC4292964 | biostudies-literature
| S-EPMC3965461 | biostudies-literature
| S-EPMC4359922 | biostudies-literature
| S-EPMC4747375 | biostudies-literature
2015-09-22 | E-GEOD-73277 | biostudies-arrayexpress
| S-EPMC3435301 | biostudies-other
| S-EPMC8806654 | biostudies-literature
| S-EPMC10215618 | biostudies-literature