Ontology highlight
ABSTRACT:
SUBMITTER: Wang L
PROVIDER: S-EPMC4695401 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Wang Li L Xie Ling L Ramachandran Srinivas S Lee YuanYu Y Yan Zhen Z Zhou Li L Krajewski Krzysztof K Liu Feng F Zhu Cheng C Chen David J DJ Strahl Brian D BD Jin Jian J Dokholyan Nikolay V NV Chen Xian X
Chemistry & biology 20150625 7
Regulatory mechanisms underlying γH2AX induction and the associated cell fate decision during DNA damage response (DDR) remain obscure. Here, we discover a bromodomain (BRD)-like module in DNA-PKcs (DNA-PKcs-BRD) that specifically recognizes H2AX acetyl-lysine 5 (K5ac) for sequential induction of γH2AX and concurrent cell fate decision(s). First, top-down mass spectrometry of radiation-phenotypic, full-length H2AX revealed a radiation-inducible, K5ac-dependent induction of γH2AX. Combined approa ...[more]