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Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates.

ABSTRACT: In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein-antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration.

SUBMITTER: Owens P

PROVIDER: S-EPMC4697139 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates.

Owens Peter P Phillipson Nigel N Perumal Jayakumar J O'Connor Gerard M GM Olivo Malini M

Biosensors 20151028 4

In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein-antibody binding on Raman linker molecules and are attribut ...[more]

PMID: 26516922

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