Ontology highlight
ABSTRACT:
SUBMITTER: Futai E
PROVIDER: S-EPMC4697183 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Futai Eugene E Osawa Satoko S Cai Tetsuo T Fujisawa Tomoya T Ishiura Shoichi S Tomita Taisuke T
The Journal of biological chemistry 20151111 1
γ-Secretase is a multisubunit membrane protein complex containing presenilin (PS1) as a catalytic subunit. Familial Alzheimer disease (FAD) mutations within PS1 were analyzed in yeast cells artificially expressing membrane-bound substrate, amyloid precursor protein, or Notch fused to Gal4 transcriptional activator. The FAD mutations, L166P and G384A (Leu-166 to Pro and Gly-384 to Ala substitution, respectively), were loss-of-function in yeast. We identified five amino acid substitutions that sup ...[more]