Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay.
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ABSTRACT:
SUBMITTER: Zhang G
PROVIDER: S-EPMC4702749 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
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Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay.
Zhang Guoqing G Cai Fangping F de Rivera Ivette Lorenzana IL Zhou Zhiyong Z Zhang Jing J Nkengasong John J Gao Feng F Yang Chunfu C
Journal of clinical microbiology 20151111 1
A multiplex allele-specific (MAS) assay has been developed for the detection of HIV-1 subtype C drug resistance mutations (DRMs). We have optimized the MAS assay to determine subtype B DRMs in dried blood spots (DBS) collected from patients on antiretroviral therapy. The new assay accurately detected DRMs, including low-abundance mutations that were often missed by Sanger sequencing. ...[more]
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