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One-step tumor detection from dynamic morphology tracking on aptamer-grafted surfaces.

ABSTRACT: In this paper, we report a one-step tumor cell detection approach based on the dynamic morphological behavior tracking of cancer cells on a ligand modified surface. Every cell on the surface was tracked in real time for several minutes immediately after seeding until these were finally attached. Cancer cells were found to be very active in the aptamer microenvironment, changing their shapes rapidly from spherical to semi-elliptical, with much flatter spread and extending pseudopods at regular intervals. When incubated on a functionalized surface, the balancing forces between cell surface molecules and the surface-bound aptamers, together with the flexibility of the membranes, caused cells to show these distinct dynamic activities and variations in their morphologies. On the other hand, healthy cells remained distinguishingly inactive on the surface over the same period. The quantitative image analysis of cell morphologies provided feature vectors that were statistically distinct between normal and cancer cells.

SUBMITTER: Mahmood MA

PROVIDER: S-EPMC4703374 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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One-step tumor detection from dynamic morphology tracking on aptamer-grafted surfaces.

Mahmood Mohammed Arif I MA Hasan Mohammad Raziul MR Khan Umair J M UJ Allen Peter B PB Kim Young-Tae YT Ellington Andrew D AD Iqbal Samir M SM

Technology 20151116 4

In this paper, we report a one-step tumor cell detection approach based on the dynamic morphological behavior tracking of cancer cells on a ligand modified surface. Every cell on the surface was tracked in real time for several minutes immediately after seeding until these were finally attached. Cancer cells were found to be very active in the aptamer microenvironment, changing their shapes rapidly from spherical to semi-elliptical, with much flatter spread and extending pseudopods at regular in ...[more]

PMID: 26753172

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Project description:Wetting plays a major role in the close interactions between liquids and solid surfaces, which can be tailored by modifying the chemistry as well as the structures of the surfaces' outermost layer. Several methodologies, such as chemical vapor deposition, physical vapor deposition, electroplating, and chemical reactions, among others, have been adopted for the alteration/modification of such interactions suitable for various applications. However, the fabrication of low-contact line-pinning hydrophobic surfaces via simple and easy methods remains an open challenge. In this work, we exploit one-step and multiple-step silicone oil (5-100 cSt) grafting on smooth silicon substrates (although the technique is suitable for other substrates), looking closely at the effect of viscosity as well as the volume and layers (one to five) of oil grafted as a function of the deposition method. Remarkably, the optimization of grafting of silicone oil fabrication results in non-wetting surfaces with extremely low contact angle hysteresis (CAH) below 1° and high contact angles (CAs) of ∼108° after a single grafting step, which is an order of magnitude smaller than the reported values of previous works on silicone oil-grafted surfaces. Moreover, the different droplet-surface interactions and pinning behavior can additionally be tailored to the specific application with CAH ranging from 1 to 20° and sliding angles between 1.5 and 60° (for droplet volumes of 3 μL), depending on the fabrication parameters adopted. In terms of roughness, all the samples (independent of the grafting parameters) showed small changes in the root-mean-square roughness below 20 nm. Lastly, stability analysis of the grafting method reported here under various conditions shows that the coating is quite stable under mechanical vibrations (bath ultrasonication) and in a chemical environment (ultrasonication in a bath of ethanol) but loses its low-pinning characteristics when exposed to saturated steam at T ∼ 99 °C. The findings presented here provide a basis for selecting the most appropriate and suitable method and parameters for silicone oil grafting aimed at low pinning and low hysteresis surfaces for specific applications.

Dataset Information

One-step tumor detection from dynamic morphology tracking on aptamer-grafted surfaces.

Publications

One-step tumor detection from dynamic morphology tracking on aptamer-grafted surfaces.

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