Rapid one-step biotinylation of biological and non-biological surfaces.
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ABSTRACT:
SUBMITTER: Henry S
PROVIDER: S-EPMC5809608 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Publications
Rapid one-step biotinylation of biological and non-biological surfaces.
Henry Stephen S Williams Eleanor E Barr Katie K Korchagina Elena E Tuzikov Alexandr A Ilyushina Natalia N Abayzeed Sidahmed A SA Webb Kevin F KF Bovin Nicolai N
Scientific reports 20180212 1
We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membrane viruses. The resultant biotinylated surface can then be used to interact with avidinylated conjugates, beads, vesicles, surfaces or cells. ...[more]
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