Unknown

Dataset Information

0

Structure-function characterization reveals new catalytic diversity in the galactose oxidase and glyoxal oxidase family.


ABSTRACT: Alcohol oxidases, including carbohydrate oxidases, have a long history of research that has generated fundamental biological understanding and biotechnological applications. Despite a long history of study, the galactose 6-oxidase/glyoxal oxidase family of mononuclear copper-radical oxidases, Auxiliary Activity Family 5 (AA5), is currently represented by only very few characterized members. Here we report the recombinant production and detailed structure-function analyses of two homologues from the phytopathogenic fungi Colletotrichum graminicola and C. gloeosporioides, CgrAlcOx and CglAlcOx, respectively, to explore the wider biocatalytic potential in AA5. EPR spectroscopy and crystallographic analysis confirm a common active-site structure vis-à-vis the archetypal galactose 6-oxidase from Fusarium graminearum. Strikingly, however, CgrAlcOx and CglAlcOx are essentially incapable of oxidizing galactose and galactosides, but instead efficiently catalyse the oxidation of diverse aliphatic alcohols. The results highlight the significant potential of prospecting the evolutionary diversity of AA5 to reveal novel enzyme specificities, thereby informing both biology and applications.

SUBMITTER: Yin DT 

PROVIDER: S-EPMC4703870 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Structure-function characterization reveals new catalytic diversity in the galactose oxidase and glyoxal oxidase family.

Yin DeLu Tyler DT   Urresti Saioa S   Lafond Mickael M   Johnston Esther M EM   Derikvand Fatemeh F   Ciano Luisa L   Berrin Jean-Guy JG   Henrissat Bernard B   Walton Paul H PH   Davies Gideon J GJ   Brumer Harry H  

Nature communications 20151218


Alcohol oxidases, including carbohydrate oxidases, have a long history of research that has generated fundamental biological understanding and biotechnological applications. Despite a long history of study, the galactose 6-oxidase/glyoxal oxidase family of mononuclear copper-radical oxidases, Auxiliary Activity Family 5 (AA5), is currently represented by only very few characterized members. Here we report the recombinant production and detailed structure-function analyses of two homologues from  ...[more]

Similar Datasets

| S-EPMC6130310 | biostudies-literature
| S-EPMC60802 | biostudies-literature
| S-EPMC4370742 | biostudies-literature
| S-EPMC2760806 | biostudies-other
| S-EPMC2762401 | biostudies-literature
| S-EPMC2323875 | biostudies-literature
| S-EPMC7877911 | biostudies-literature
| S-EPMC4072685 | biostudies-literature
| S-EPMC7183269 | biostudies-literature
| S-EPMC3374809 | biostudies-literature