Visualisation of newly synthesised collagen in vitro and in vivo.
Ontology highlight
ABSTRACT:
SUBMITTER: Oostendorp C
PROVIDER: S-EPMC4704054 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Publications
Visualisation of newly synthesised collagen in vitro and in vivo.
Oostendorp Corien C Uijtdewilligen Peter J E PJ Versteeg Elly M EM Hafmans Theo G TG van den Bogaard Ellen H EH de Jonge Paul K J D PK Pirayesh Ali A Von den Hoff Johannes W JW Reichmann Ernst E Daamen Willeke F WF van Kuppevelt Toin H TH
Scientific reports 20160107
Identifying collagen produced de novo by cells in a background of purified collagenous biomaterials poses a major problem in for example the evaluation of tissue-engineered constructs and cell biological studies to tumor dissemination. We have developed a universal strategy to detect and localize newly deposited collagen based on its inherent association with dermatan sulfate. The method is applicable irrespective of host species and collagen source. ...[more]
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