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Development of an Intracellular Screen for New Compounds Able To Inhibit Mycobacterium tuberculosis Growth in Human Macrophages.

ABSTRACT: Here we describe the development and validation of an intracellular high-throughput screening assay for finding new antituberculosis compounds active in human macrophages. The assay consists of a luciferase-based primary identification assay, followed by a green fluorescent protein-based secondary profiling assay. Standard tuberculosis drugs and 158 previously recognized active antimycobacterial compounds were used to evaluate assay robustness. Data show that the assay developed is a short and valuable tool for the discovery of new antimycobacterial compounds.

SUBMITTER: Sorrentino F

PROVIDER: S-EPMC4704166 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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Development of an Intracellular Screen for New Compounds Able To Inhibit Mycobacterium tuberculosis Growth in Human Macrophages.

Sorrentino Flavia F Gonzalez del Rio Ruben R Zheng Xingji X Presa Matilla Jesus J Torres Gomez Pedro P Martinez Hoyos Maria M Perez Herran Maria Esther ME Mendoza Losana Alfonso A Av-Gay Yossef Y

Antimicrobial agents and chemotherapy 20151026 1

Here we describe the development and validation of an intracellular high-throughput screening assay for finding new antituberculosis compounds active in human macrophages. The assay consists of a luciferase-based primary identification assay, followed by a green fluorescent protein-based secondary profiling assay. Standard tuberculosis drugs and 158 previously recognized active antimycobacterial compounds were used to evaluate assay robustness. Data show that the assay developed is a short and v ...[more]

PMID: 26503663

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