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FosI Is a New Integron-Associated Gene Cassette Encoding Reduced Susceptibility to Fosfomycin.

ABSTRACT: In this work, we demonstrate that the fosI gene encodes a predicted small protein with 134 amino acids and determines reduced susceptibility to fosfomycin. It raised the MIC from 0.125 to 6 ?g/ml when the pBRA100 plasmid was introduced into Escherichia coli TOP10 and to 16 ?g/ml when the gene was cloned into the pBC_SK(-) vector and expressed in E. coli TOP10.

SUBMITTER: Pelegrino Kde O

PROVIDER: S-EPMC4704179 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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fosI Is a New Integron-Associated Gene Cassette Encoding Reduced Susceptibility to Fosfomycin.

Pelegrino Karla de Oliveira Kde O Campos Juliana Coutinho JC Sampaio Suely Carlos Ferreira SC Lezirovitz Karina K Seco Bruna Mara BM Pereira Mayne de Oliveira Mde O Rocha Darlan Augusto da Costa DA Jové Thomas T Nicodemo Antonio Carlos AC Sampaio Jorge Luiz Mello JL

Antimicrobial agents and chemotherapy 20151109 1

In this work, we demonstrate that the fosI gene encodes a predicted small protein with 134 amino acids and determines reduced susceptibility to fosfomycin. It raised the MIC from 0.125 to 6 μg/ml when the pBRA100 plasmid was introduced into Escherichia coli TOP10 and to 16 μg/ml when the gene was cloned into the pBC_SK(-) vector and expressed in E. coli TOP10. ...[more]

PMID: 26552984

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