Ontology highlight
ABSTRACT:
SUBMITTER: Wang Q
PROVIDER: S-EPMC4706425 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Wang Qiang Q Waterhouse Nicklas N Feyijinmi Olusegun O Dominguez Matthew J MJ Martinez Lisa M LM Sharp Zoey Z Service Rachel R Bothe Jameson R JR Stollar Elliott J EJ
PloS one 20160108 1
The kinetics of folding and unfolding underlie protein stability and quantification of these rates provides important insights into the folding process. Here, we present a simple high throughput protein unfolding kinetic assay using a plate reader that is applicable to the studies of the majority of 2-state folding proteins. We validate the assay by measuring kinetic unfolding data for the SH3 (Src Homology 3) domain from Actin Binding Protein 1 (AbpSH3) and its stabilized mutants. The results o ...[more]