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Biomimetic RNA-silencing nanocomplexes: overcoming multidrug resistance in cancer cells.

ABSTRACT: RNA interference (RNAi) is an RNA-dependent gene silencing approach controlled by an RNA-induced silencing complex (RISC). Herein, we present a synthetic RISC-mimic nanocomplex, which can actively cleave its target RNA in a sequence-specific manner. With high enzymatic stability and efficient self-delivery to target cells, the designed nanocomplex can selectively and potently induce gene silencing without cytokine activation. These nanocomplexes, which target multidrug resistance, are not only able to bypass the P-glycoprotein (Pgp) transporter, due to their nano-size effect, but also effectively suppress Pgp expression, thus resulting in successful restoration of drug sensitivity of OVCAR8/ADR cells to Pgp-transportable cytotoxic agents. This nanocomplex approach has the potential for both functional genomics and cancer therapy.

SUBMITTER: Wang Z

PROVIDER: S-EPMC4709245 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

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Biomimetic RNA-silencing nanocomplexes: overcoming multidrug resistance in cancer cells.

Wang Zhongliang Z Wang Zhe Z Liu Dingbin D Yan Xuefeng X Wang Fu F Niu Gang G Yang Min M Chen Xiaoyuan X

Angewandte Chemie (International ed. in English) 20140120 7

RNA interference (RNAi) is an RNA-dependent gene silencing approach controlled by an RNA-induced silencing complex (RISC). Herein, we present a synthetic RISC-mimic nanocomplex, which can actively cleave its target RNA in a sequence-specific manner. With high enzymatic stability and efficient self-delivery to target cells, the designed nanocomplex can selectively and potently induce gene silencing without cytokine activation. These nanocomplexes, which target multidrug resistance, are not only a ...[more]

PMID: 24446433

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