Ontology highlight
ABSTRACT:
SUBMITTER: Lawrence RM
PROVIDER: S-EPMC4711640 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Lawrence Robert M RM Conrad Chelsie E CE Zatsepin Nadia A NA Grant Thomas D TD Liu Haiguang H James Daniel D Nelson Garrett G Subramanian Ganesh G Aquila Andrew A Hunter Mark S MS Liang Mengning M Boutet Sébastien S Coe Jesse J Spence John C H JC Weierstall Uwe U Liu Wei W Fromme Petra P Cherezov Vadim V Hogue Brenda G BG
Structural dynamics (Melville, N.Y.) 20150701 4
Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ∼700 Å diameter. Microcrystals delivered in viscous agarose medium diffracted to ∼40 Å resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins un ...[more]