Ontology highlight
ABSTRACT:
SUBMITTER: Buß O
PROVIDER: S-EPMC4711668 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
PloS one 20160105 1
β-keto esters are used as precursors for the synthesis of β-amino acids, which are building blocks for some classes of pharmaceuticals. Here we describe the comparison of screening procedures for hydrolases to be used for the hydrolysis of β-keto esters, the first step in the preparation of β-amino acids. Two of the tested high throughput screening (HTS) assays depend on coupled enzymatic reactions which detect the alcohol released during ester hydrolysis by luminescence or absorption. The third ...[more]