Ontology highlight
ABSTRACT:
SUBMITTER: Helgadottir S
PROVIDER: S-EPMC4712913 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Helgadóttir Sunna S Sinapah Sylvie S Söll Dieter D Ling Jiqiang J
FEBS letters 20111209 1
In methanogenic archaea, Sep-tRNA:Cys-tRNA synthase (SepCysS) converts Sep-tRNA(Cys) to Cys-tRNA(Cys). The mechanism of tRNA-dependent cysteine formation remains unclear due to the lack of functional studies. In this work, we mutated 19 conserved residues in Methanocaldococcus jannaschii SepCysS, and employed an in vivo system to determine the activity of the resulting variants. Our results show that three active-site cysteines (Cys39, Cys42 and Cys247) are essential for SepCysS activity. In add ...[more]