Development of a high-copy plasmid for enhanced production of recombinant proteins in Leuconostoc citreum.

ABSTRACT:

Background

Leuconostoc is a hetero-fermentative lactic acid bacteria, and its importance is widely recognized in the dairy industry. However, due to limited genetic tools including plasmids for Leuconostoc, there has not been much extensive research on the genetics and engineering of Leuconostoc yet. Thus, there is a big demand for high-copy-number plasmids for useful gene manipulation and overproduction of recombinant proteins in Leuconostoc.

Results

Using an existing low-copy plasmid, the copy number of plasmid was increased by random mutagenesis followed by FACS-based high-throughput screening. First, a random library of plasmids was constructed by randomizing the region responsible for replication in Leuconostoc citreum; additionally, a superfolder green fluorescent prot

SUBMITTER: Son YJ 

PROVIDER: S-EPMC4714500 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature