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Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies.

ABSTRACT: To isolate specific genomic regions that retain their molecular interactions, allowing direct identification of chromatin-bound molecules, we developed two locus-specific chromatin immunoprecipitation (locus-specific ChIP) technologies, insertional ChIP (iChIP) and engineered DNA-binding molecule-mediated ChIP (enChIP) using the clustered regularly interspaced short palindromic repeats (CRISPR) system or transcription activator-like (TAL) proteins. Essentially, a locus-specific ChIP consists of locus-tagging and affinity purification and can be combined with downstream analyses to identify molecules associated with the target genomic regions. In this review, we discuss the applications of locus-specific ChIP to analyze the genome functions, including transcription and epigenetic regulation.

SUBMITTER: Fujita T

PROVIDER: S-EPMC4718151 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies.

Fujita Toshitsugu T Fujii Hodaka H

Gene regulation and systems biology 20160118 Suppl 1

To isolate specific genomic regions that retain their molecular interactions, allowing direct identification of chromatin-bound molecules, we developed two locus-specific chromatin immunoprecipitation (locus-specific ChIP) technologies, insertional ChIP (iChIP) and engineered DNA-binding molecule-mediated ChIP (enChIP) using the clustered regularly interspaced short palindromic repeats (CRISPR) system or transcription activator-like (TAL) proteins. Essentially, a locus-specific ChIP consists of ...[more]

PMID: 26819551

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