Dataset Information

Identification of the Sfp-Type PPTase EppA from the Lichenized Fungus Evernia prunastri.

ABSTRACT: In the last decades, natural products from lichens have gained more interest for pharmaceutical application due to the broad range of their biological activity. However, isolation of the compounds of interest directly from the lichen is neither feasible nor sustainable due to slow growth of many lichens. In order to develop a pipeline for heterologous expression of lichen biosynthesis gene clusters and thus the sustainable production of their bioactive compounds we have identified and characterized the phosphopantheteinyl transferase (PPTase) EppA from the lichen Evernia prunastri. The Sfp-type PPTase EppA was functionally characterized through heterologous expression in E. coli using the production of the blue pigment indigoidine as readout and by complementation of a lys5 deletion in S. cerevisiae.

SUBMITTER: Schimming O

PROVIDER: S-EPMC4718654 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Identification of the Sfp-Type PPTase EppA from the Lichenized Fungus Evernia prunastri.

Schimming Olivia O Schmitt Imke I Bode Helge B HB

PloS one 20160119 1

In the last decades, natural products from lichens have gained more interest for pharmaceutical application due to the broad range of their biological activity. However, isolation of the compounds of interest directly from the lichen is neither feasible nor sustainable due to slow growth of many lichens. In order to develop a pipeline for heterologous expression of lichen biosynthesis gene clusters and thus the sustainable production of their bioactive compounds we have identified and characteri ...[more]

PMID: 26784935

Similar Datasets

Project description:Background and aimsOnset of reproduction and reproductive allocation patterns are key components of plant reproductive strategies. Life history theory predicts that plants in adverse environments for juvenile performance start reproduction at smaller sizes and exhibit higher reproductive allocation compared to their counterparts in favourable environments. Life history theory will gain in generality if its predictions are shown to apply to a broad range of organisms and modes of reproduction. This study tested whether the asexual reproductive strategy of a lichenized fungus changed along a climatic gradient.MethodsThe variation in threshold size for asexual reproduction and asexual reproductive allocation of the lichen Lobarina scrobiculata was assessed in 18 populations (9665 individuals) along a climatic gradient spanning 800?km in latitude in Southern Europe. Using generalized linear models and standardized major axis regressions, the allometric relationships and the associated variation in climatic factors according to the changes in the threshold size for reproduction and reproductive allocation patterns were assessed.Key resultsThe onset of reproduction was size-dependent and the reproductive allocation increased with individual size. Both the threshold size for reproduction and the reproductive allocation varied along the rainfall gradient. A lower threshold size for reproduction and higher reproductive allocation in drier, adverse locations were found. Therefore, populations in drier locations fitted the predictions of life history theory for sexually reproducing organisms in adverse environments for juvenile performance.ConclusionsThis study highlights the applicability of the life history theory to fungi and to modes of reproduction other than sexual reproduction. Based on the intraspecific variation in the asexual reproductive strategy of a fungal organism with climatic factors, these findings expand the scope of life history theory predictions and increase our understanding of life history diversity and reproductive strategies across environments.

Dataset Information

Identification of the Sfp-Type PPTase EppA from the Lichenized Fungus Evernia prunastri.

Publications

Identification of the Sfp-Type PPTase EppA from the Lichenized Fungus Evernia prunastri.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets