Ontology highlight
ABSTRACT:
SUBMITTER: Lu F
PROVIDER: S-EPMC4718804 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Lu Feiran F Liang Qiren Q Abi-Mosleh Lina L Das Akash A De Brabander Jef K JK Goldstein Joseph L JL Brown Michael S MS
eLife 20151208
Niemann-Pick C1 (NPC1) is a lysosomal membrane protein that exports cholesterol derived from receptor-mediated uptake of LDL, and it also mediates cellular entry of Ebola virus. Cholesterol export is inhibited by nanomolar concentrations of U18666A, a cationic sterol. To identify the target of U18666A, we synthesized U-X, a U18666A derivative with a benzophenone that permits ultraviolet-induced crosslinking. When added to CHO cells, U-X crosslinked to NPC1. Crosslinking was blocked by U18666A de ...[more]