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Partial denture metal framework may harbor potentially pathogenic bacteria.

ABSTRACT: PURPOSE:The aim of this study was to characterize and compare bacterial diversity on the removable partial denture (RPD) framework over time. MATERIALS AND METHODS:This descriptive pilot study included five women who were rehabilitated with free-end mandibular RPD. The biofilm on T-bar clasps were collected 1 week (t1) and 4 months (t2) after the RPD was inserted (t0). Bacterial 16S rDNA was extracted and PCR amplified. Amplicons were cloned; clones were submitted to cycle sequencing, and sequences were compared with GenBank (98% similarity). RESULTS:A total of 180 sequences with more than 499 bp were obtained. Two phylogenetic trees with 84 (t1) and 96 (t2) clones represented the bacteria biofilm at the RPD. About 93% of the obtained phylotypes fell into 25 known species for t1 and 17 for t2, which were grouped in 5 phyla: Firmicutes (t1=82%; t2=60%), Actinobacteria (t1=5%; t2=10%), Bacteroidetes (t1=2%; t2=6%), Proteobacteria (t1=10%; t2=15%) and Fusobacteria (t1=1%; t2=8%). The libraries also include 3 novel phylotypes for t1 and 11 for t2. Library t2 differs from t1 (P=.004); t1 is a subset of the t2 (P=.052). Periodontal pathogens, such as F. nucleatum, were more prevalent in t2. CONCLUSION:The biofilm composition of the RPD metal clasps changed along time after RPD wearing. The RPD framework may act as a reservoir for potentially pathogenic bacteria and the RPD wearers may benefit from regular follow-up visits and strategies on prosthesis-related oral health instructions.

SUBMITTER: Mengatto CM

PROVIDER: S-EPMC4722151 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Partial denture metal framework may harbor potentially pathogenic bacteria.

Mengatto Cristiane Machado CM Marchini Leonardo L Bernardes Luciano Angelo de Souza LA Gomes Sabrina Carvalho SC Silva Alecsandro Moura AM Rizzatti-Barbosa Célia Marisa CM Rizzatti-Barbosa Célia Marisa CM

The journal of advanced prosthodontics 20151230 6

<h4>Purpose</h4>The aim of this study was to characterize and compare bacterial diversity on the removable partial denture (RPD) framework over time.<h4>Materials and methods</h4>This descriptive pilot study included five women who were rehabilitated with free-end mandibular RPD. The biofilm on T-bar clasps were collected 1 week (t1) and 4 months (t2) after the RPD was inserted (t0). Bacterial 16S rDNA was extracted and PCR amplified. Amplicons were cloned; clones were submitted to cycle sequenc ...[more]

PMID: 26816577

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Partial denture metal framework may harbor potentially pathogenic bacteria.

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Partial denture metal framework may harbor potentially pathogenic bacteria.

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