Ontology highlight
ABSTRACT:
SUBMITTER: De Paoli HC
PROVIDER: S-EPMC4725820 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Scientific reports 20160113
Successful synthetic biology efforts rely on conceptual and experimental designs in combination with testing of multi-gene constructs. Despite recent progresses, several limitations still hinder the ability to flexibly assemble and collectively share different types of DNA segments. Here, we describe an advanced system for joining DNA fragments from a universal library that automatically maintains open reading frames (ORFs) and does not require linkers, adaptors, sequence homology, amplification ...[more]