Ontology highlight
ABSTRACT:
SUBMITTER: Coto Hernandez I
PROVIDER: S-EPMC4725939 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Scientific reports 20160113
We report on a novel two-photon excitation stimulated emission depletion (2PE-STED) microscope based on time-gated detection. The time-gated detection allows for the effective silencing of the fluorophores using moderate stimulated emission beam intensity. This opens the possibility of implementing an efficient 2PE-STED microscope with a stimulated emission beam running in a continuous-wave. The continuous-wave stimulated emission beam tempers the laser architecture's complexity and cost, but th ...[more]