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Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue.

ABSTRACT: Multifocal structured illumination microscopy (MSIM) provides a twofold resolution enhancement beyond the diffraction limit at sample depths up to 50 µm, but scattered and out-of-focus light in thick samples degrades MSIM performance. Here we implement MSIM with a microlens array to enable efficient two-photon excitation. Two-photon MSIM gives resolution-doubled images with better sectioning and contrast in thick scattering samples such as Caenorhabditis elegans embryos, Drosophila melanogaster larval salivary glands, and mouse liver tissue.

SUBMITTER: Ingaramo M

PROVIDER: S-EPMC3986197 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue.

Ingaramo Maria M York Andrew G AG Wawrzusin Peter P Milberg Oleg O Hong Amy A Weigert Roberto R Shroff Hari H Patterson George H GH

Proceedings of the National Academy of Sciences of the United States of America 20140324 14

Multifocal structured illumination microscopy (MSIM) provides a twofold resolution enhancement beyond the diffraction limit at sample depths up to 50 µm, but scattered and out-of-focus light in thick samples degrades MSIM performance. Here we implement MSIM with a microlens array to enable efficient two-photon excitation. Two-photon MSIM gives resolution-doubled images with better sectioning and contrast in thick scattering samples such as Caenorhabditis elegans embryos, Drosophila melanogaster ...[more]

PMID: 24706872

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