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First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes.

ABSTRACT: This study evaluated the antifungal susceptibility profile and the production of potential virulence attributes in a clinical strain of Candida nivariensis for the first time in Brazil, as identified by sequencing the internal transcribed spacer (ITS)1-5.8S-ITS2 region and D1/D2 domains of the 28S of the rDNA. For comparative purposes, tests were also performed with reference strains. All strains presented low planktonic minimal inhibitory concentrations (PMICs) to amphotericin B (AMB), caspofungin (CAS), and voriconazole. However, our strain showed elevated planktonic MICs to posaconazole (POS) and itraconazole, in addition to fluconazole resistance. Adherence to inert surfaces was conducted onto glass and polystyrene. The biofilm formation and antifungal susceptibility on biofilm-growing cells were evaluated by crystal violet staining and a XTT reduction assay. All fungal strains were able to bind both tested surfaces and form biofilm, with a binding preference to polystyrene (p < 0.001). AMB promoted significant reductions (?50%) in biofilm production by our C. nivariensis strain using both methodologies. This reduction was also observed for CAS and POS, but only in the XTT assay. All strains were excellent protease producers and moderate phytase producers, but lipases were not detected. This study reinforces the pathogenic potential of C. nivariensis and its possible resistance profile to the azolic drugs generally used for candidiasis management.

SUBMITTER: Figueiredo-Carvalho MH

PROVIDER: S-EPMC4727436 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes.

Figueiredo-Carvalho Maria Helena Galdino MH Ramos Livia de Souza Lde S Barbedo Leonardo Silva LS Chaves Alessandra Leal da Silva AL Muramoto Ilda Akemi IA Santos André Luis Souza dos AL Almeida-Paes Rodrigo R Zancopé-Oliveira Rosely Maria RM

Memorias do Instituto Oswaldo Cruz 20160101 1

This study evaluated the antifungal susceptibility profile and the production of potential virulence attributes in a clinical strain of Candida nivariensis for the first time in Brazil, as identified by sequencing the internal transcribed spacer (ITS)1-5.8S-ITS2 region and D1/D2 domains of the 28S of the rDNA. For comparative purposes, tests were also performed with reference strains. All strains presented low planktonic minimal inhibitory concentrations (PMICs) to amphotericin B (AMB), caspofun ...[more]

PMID: 26814644

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Dataset Information

First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes.

Publications

First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes.

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