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Photonic Crystal Optical Tweezers with High Efficiency for Live Biological Samples and Viability Characterization.

ABSTRACT: We propose and demonstrate a new optical trapping method for single cells that utilizes modulated light fields to trap a wide array of cell types, including mammalian, yeast, and Escherichia coli cells, on the surface of a two-dimensional photonic crystal. This method is capable of reducing the required light intensity, and thus minimizing the photothermal damage to living cells, thereby extending cell viability in optical trapping and cell manipulation applications. To this end, a thorough characterization of cell viability in optical trapping environments was performed. This study also demonstrates the technique using spatial light modulation in patterned manipulation of live cell arrays over a broad area.

SUBMITTER: Jing P

PROVIDER: S-EPMC4728687 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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Photonic Crystal Optical Tweezers with High Efficiency for Live Biological Samples and Viability Characterization.

Jing Peifeng P Wu Jingda J Liu Gary W GW Keeler Ethan G EG Pun Suzie H SH Lin Lih Y LY

Scientific reports 20160127

We propose and demonstrate a new optical trapping method for single cells that utilizes modulated light fields to trap a wide array of cell types, including mammalian, yeast, and Escherichia coli cells, on the surface of a two-dimensional photonic crystal. This method is capable of reducing the required light intensity, and thus minimizing the photothermal damage to living cells, thereby extending cell viability in optical trapping and cell manipulation applications. To this end, a thorough char ...[more]

PMID: 26814808

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