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Chemical Proteomic Platform To Identify Citrullinated Proteins.

ABSTRACT: Anti-citrullinated protein antibodies (ACPAs) are a hallmark of rheumatoid arthritis (RA) and are routinely used for disease diagnosis. Protein citrullination is also increased in cancer and other autoimmune disorders, suggesting that citrullinated proteins may serve as biomarkers for diseases beyond RA. To identify these citrullinated proteins, we developed biotin-conjugated phenylglyoxal (biotin-PG). Using this probe and our platform technology, we identified >50 intracellular citrullinated proteins. More than 20 of these are involved in RNA splicing, suggesting, for the first time, that citrullination modulates RNA biology. Overall, this chemical proteomic platform will play a key role in furthering our understanding of protein citrullination in rheumatoid arthritis and potentially a wider spectrum of inflammatory diseases.

SUBMITTER: Lewallen DM

PROVIDER: S-EPMC4729336 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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Chemical Proteomic Platform To Identify Citrullinated Proteins.

Lewallen Daniel M DM Bicker Kevin L KL Subramanian Venkataraman V Clancy Kathleen W KW Slade Daniel J DJ Martell Julianne J Dreyton Christina J CJ Sokolove Jeremy J Weerapana Eranthie E Thompson Paul R PR

ACS chemical biology 20150923 11

Anti-citrullinated protein antibodies (ACPAs) are a hallmark of rheumatoid arthritis (RA) and are routinely used for disease diagnosis. Protein citrullination is also increased in cancer and other autoimmune disorders, suggesting that citrullinated proteins may serve as biomarkers for diseases beyond RA. To identify these citrullinated proteins, we developed biotin-conjugated phenylglyoxal (biotin-PG). Using this probe and our platform technology, we identified >50 intracellular citrullinated pr ...[more]

PMID: 26360112

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