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Slow DNA transport through nanopores in hafnium oxide membranes.

ABSTRACT: We present a study of double- and single-stranded DNA transport through nanopores fabricated in ultrathin (2-7 nm thick) freestanding hafnium oxide (HfO2) membranes. The high chemical stability of ultrathin HfO2 enables long-lived experiments with <2 nm diameter pores that last several hours, in which we observe >50 000 DNA translocations with no detectable pore expansion. Mean DNA velocities are slower than velocities through comparable silicon nitride pores, providing evidence that HfO2 nanopores have favorable physicochemical interactions with nucleic acids that can be leveraged to slow down DNA in a nanopore.

SUBMITTER: Larkin J

PROVIDER: S-EPMC4729694 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Slow DNA transport through nanopores in hafnium oxide membranes.

Larkin Joseph J Henley Robert R Bell David C DC Cohen-Karni Tzahi T Rosenstein Jacob K JK Wanunu Meni M

ACS nano 20131004 11

We present a study of double- and single-stranded DNA transport through nanopores fabricated in ultrathin (2-7 nm thick) freestanding hafnium oxide (HfO2) membranes. The high chemical stability of ultrathin HfO2 enables long-lived experiments with <2 nm diameter pores that last several hours, in which we observe >50 000 DNA translocations with no detectable pore expansion. Mean DNA velocities are slower than velocities through comparable silicon nitride pores, providing evidence that HfO2 nanopo ...[more]

PMID: 24083444

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