Ontology highlight
ABSTRACT:
SUBMITTER: Jin SG
PROVIDER: S-EPMC4731272 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Jin Seung-Gi SG Zhang Zhi-Min ZM Dunwell Thomas L TL Harter Matthew R MR Wu Xiwei X Johnson Jennifer J Li Zheng Z Liu Jiancheng J Szabó Piroska E PE Lu Qiang Q Xu Guo-Liang GL Song Jikui J Pfeifer Gerd P GP
Cell reports 20160107 3
We report that the mammalian 5-methylcytosine (5mC) oxidase Tet3 exists as three major isoforms and characterized the full-length isoform containing an N-terminal CXXC domain (Tet3FL). This CXXC domain binds to unmethylated CpGs, but, unexpectedly, its highest affinity is toward 5-carboxylcytosine (5caC). We determined the crystal structure of the CXXC domain-5caC-DNA complex, revealing the structural basis of the binding specificity of this domain as a reader of CcaCG sequences. Mapping of Tet3 ...[more]