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Donor-derived exosomes induce specific regulatory T cells to suppress immune inflammation in the allograft heart.

ABSTRACT: To inhibit the immune inflammation in the allografts can be beneficial to organ transplantation. This study aims to induce the donor antigen specific regulatory T cells (Treg cell) inhibit the immune inflammation in the allograft heart. In this study, peripheral exosomes were purified from the mouse serum. A heart transplantation mouse model was developed. The immune inflammation of the allograft heart was assessed by histology and flow cytometry. The results showed that the donor antigen-specific T helper (Th)2 pattern inflammation was observed in the allograft hearts; the inflammation was inhibited by immunizing the recipient mice with the donor-derived exosomes. Purified peripheral exosomes contained integrin MMP1a; the latter induced CD4(+) T cells to express Fork head protein-3 and transforming growth factor (TGF)-β via inhibiting the Th2 transcription factor, GATA binding protein 3, in CD4(+) T cells. Administration with the donor-derived exosomes significantly prolonged the allograft heart survival. We conclude that the donor-derived peripheral exosomes have the capacity to inhibit the immune inflammation in the allograft heart via inducing specific Treg cells, implicating that administration with the donor-derived exosomes may be beneficial to cardiac transplantation.

SUBMITTER: Song J

PROVIDER: S-EPMC4731812 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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Donor-derived exosomes induce specific regulatory T cells to suppress immune inflammation in the allograft heart.

Song Jiangping J Huang Jie J Chen Xiao X Teng Xiao X Song Zhizhao Z Xing Yong Y Wang Mangyuan M Chen Kai K Wang Zheng Z Yang Pingchang P Hu Shengshou S

Scientific reports 20160129

To inhibit the immune inflammation in the allografts can be beneficial to organ transplantation. This study aims to induce the donor antigen specific regulatory T cells (Treg cell) inhibit the immune inflammation in the allograft heart. In this study, peripheral exosomes were purified from the mouse serum. A heart transplantation mouse model was developed. The immune inflammation of the allograft heart was assessed by histology and flow cytometry. The results showed that the donor antigen-specif ...[more]

PMID: 26822278

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Donor-derived exosomes induce specific regulatory T cells to suppress immune inflammation in the allograft heart.

Publications

Donor-derived exosomes induce specific regulatory T cells to suppress immune inflammation in the allograft heart.

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