Unknown

Dataset Information

0

Cardiac endothelial cell-derived exosomes induce specific regulatory B cells.


ABSTRACT: The mechanism of immune tolerance is to be further understood. The present study aims to investigate the role of the Cardiac endothelial cell (CEC)-derived exosomes in the induction of regulatory B cells. In this study, CECs were isolated from the mouse heart. Exosomes were purified from the culture supernatant of the primary endothelial cells. The suppressor functions of the regulatory B cells were determined by flow cytometry. The results showed that the CEC-derived exosomes carried integrin αvβ6. Exposure to lipopolysaccharide (LPS) induced B cells to express the latent transforming growth factor (TGF)-β, the latter was converted to the active form, TGF-β, by the exosome-derived αvβ6. The B cells released TGF-β in response to re-exposure to the exosomes in the culture, which suppressed the effector T cell proliferation. We conclude that CEC-derived exosomes have the capacity to induce B cells with immune suppressor functions.

SUBMITTER: Song J 

PROVIDER: S-EPMC4274510 | biostudies-other | 2014

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC8102743 | biostudies-literature
| S-EPMC4731812 | biostudies-literature
| S-EPMC4991413 | biostudies-literature
| S-EPMC9534701 | biostudies-literature
| S-EPMC6239974 | biostudies-literature
| S-EPMC5556362 | biostudies-other
| S-EPMC8841477 | biostudies-literature
| S-EPMC5986551 | biostudies-literature
| S-EPMC6465166 | biostudies-literature
| S-EPMC3951359 | biostudies-literature