Ontology highlight
ABSTRACT:
SUBMITTER: Hanswillemenke A
PROVIDER: S-EPMC4731850 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Hanswillemenke Alfred A Kuzdere Tahsin T Vogel Paul P Jékely Gáspár G Stafforst Thorsten T
Journal of the American Chemical Society 20151210 50
Site-directed RNA editing allows for the manipulation of RNA and protein function by reprogramming genetic information at the RNA level. For this we assemble artificial RNA-guided editases and demonstrate their transcript repair activity in cells and in developing embryos of the annelid Platynereis dumerilii. A hallmark of our assembly strategy is the covalent attachment of guideRNA and editing enzyme by applying the SNAP-tag technology, a process that we demonstrate here to be readily triggered ...[more]