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Serological, genomic and structural analyses of the major mite allergen Der p 23.


ABSTRACT: BACKGROUND:Der p 23 was recently identified in a European population as a major allergen and potentially a chitin binding protein. OBJECTIVE:This study sought to assess the importance of Der p 23 among other Dermatophagoides allergens in a North American population and to determine the structure for functional characterization. METHODS:IgE binding to Der p 23, Der p 1, Der p 2, Der p 5, Der p 7 and Der p 8 was measured by ELISA. RNA-seq data from D. pteronyssinus were compared as estimates of allergen expression levels. The structure was analysed by X-ray crystallography and NMR. RESULTS:Despite a high prevalence of Der p 23, (75% vs. 87% and 79% for Der p 1 and Der p 2, respectively), the anti-Der p 23 IgE levels were relatively low. The patient response to the 6 allergens tested was variable (n = 47), but on average anti-Der p 1 and anti-Der p 2 together accounted for 85% of the specific IgE. In terms of abundance, the RNA expression level of Der p 23 is the lowest of the major allergens, thirty fold less than Der p 1 and sevenfold less than Der p 2. The structure of Der p 23 is a small, globular protein stabilized by two disulphide bonds, which is structurally related to allergens such as Blo t 12 that contain carbohydrate binding domains that bind chitin. Functional assays failed to confirm chitin binding by Der p 23. CONCLUSIONS AND CLINICAL RELEVANCE:Der p 23 accounts for a small percentage of the IgE response to mite allergens, which is dominated by Der p 1 and Der p 2. The prevalence and amount of specific IgE to Der p 23 and Der p 2 are disproportionately high compared to the expression of other Dermatophagoides allergens.

SUBMITTER: Mueller GA 

PROVIDER: S-EPMC4732897 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Serological, genomic and structural analyses of the major mite allergen Der p 23.

Mueller G A GA   Randall T A TA   Glesner J J   Pedersen L C LC   Perera L L   Edwards L L LL   DeRose E F EF   Chapman M D MD   London R E RE   Pomés A A  

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 20160201 2


<h4>Background</h4>Der p 23 was recently identified in a European population as a major allergen and potentially a chitin binding protein.<h4>Objective</h4>This study sought to assess the importance of Der p 23 among other Dermatophagoides allergens in a North American population and to determine the structure for functional characterization.<h4>Methods</h4>IgE binding to Der p 23, Der p 1, Der p 2, Der p 5, Der p 7 and Der p 8 was measured by ELISA. RNA-seq data from D. pteronyssinus were compa  ...[more]

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