Dataset Information

Transcriptome and Expression Patterns of Chemosensory Genes in Antennae of the Parasitoid Wasp Chouioia cunea.

ABSTRACT: Chouioia cunea Yang is an endoparasitic wasp that attacks pupae of Hyphantria cunea (Drury), an invasive moth species that severely damages forests in China. Chemosensory systems of insects are used to detect volatile chemical odors such as female sex pheromones and host plant volatiles. The antennae of parasite wasps are important for host detection and other sensory-mediated behaviors. We identified and documented differential expression profiles of chemoreception genes in C. cunea antennae. A total of 25 OBPs, 80 ORs, 10 IRs, 11 CSP, 1 SNMPs, and 17 GRs were annotated from adult male and female C. cunea antennal transcriptomes. The expression profiles of 25 OBPs, 16 ORs, and 17 GRs, 5 CSP, 5 IRs and 1 SNMP were determined by RT-PCR and RT-qPCR for the antenna, head, thorax, and abdomen of male and female C. cunea. A total of 8 OBPs, 14 ORs, and 8 GRs, 1 CSP, 4 IRs and 1 SNMPs were exclusively or primarily expressed in female antennae. These female antennal-specific or dominant expression profiles may assist in locating suitable host and oviposition sites. These genes will provide useful targets for advanced study of their biological functions.

SUBMITTER: Zhao Y

PROVIDER: S-EPMC4739689 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Transcriptome and Expression Patterns of Chemosensory Genes in Antennae of the Parasitoid Wasp Chouioia cunea.

Zhao Yanni Y Wang Fengzhu F Zhang Xinyue X Zhang Suhua S Guo Shilong S Zhu Gengping G Liu Qiang Q Li Min M

PloS one 20160203 2

Chouioia cunea Yang is an endoparasitic wasp that attacks pupae of Hyphantria cunea (Drury), an invasive moth species that severely damages forests in China. Chemosensory systems of insects are used to detect volatile chemical odors such as female sex pheromones and host plant volatiles. The antennae of parasite wasps are important for host detection and other sensory-mediated behaviors. We identified and documented differential expression profiles of chemoreception genes in C. cunea antennae. A ...[more]

PMID: 26841106

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Project description:Chouioia cunea (Yang) is an endoparasitic wasp which parasitizes pupae and thus plays an important role in the biological control of the fall webworm (Hyphantria cunea Drury), an important quarantine pest in the entire world and a major invasive pest in China. For the purposes of investigating which proteins are involved in the response of C. cunea to 1-Docecene, one of the chemical compounds of pupae of H. cunea with a significant attracting action to mated female C. cunea, 11.5?Gb transcriptome data was sequenced on the PacBio RS II platform from 1-day old C. cunea adults to generate a reference assembly. Afterwards, 46.88?Gb of clean RNA-Seq data were obtained to assess the transcriptional response of these insects before and after the stimulation with 1-Docecene. After removing redundancy using CD-HIT, a sequence structure analysis predicted 29,105 complete coding sequence (CDS) regions, 51,458 single-sequence repeats (SSRs), and 2,375 long non-coding RNAs. Based on the early transcriptome sequencing in our laboratory, we revealed some new sequences corresponding to chemosensory genes such as odorant binding proteins (OBPs), odorant receptor (OR), gustatory receptors(GRs). Results of quantitative real-time PCR experiments revealed that CcOBP7, CcOBP18, CcCSP4, CcOR2, and CcGR18 were up-regulated after 1-Dodecene stimulation. In addition, the expression of 31 genes, including 1 gene related to phospholipid biosynthesis and 2 genes related to transmembrane transport were up-regulated after 1-Dodecene stimulation; meanwhile, the expression of 22 genes, including 5 genes related to protein phosphorylation and protein serine/threonine kinase activity were significantly down-regulated after 1-Dodecene stimulation. These results suggest that the attraction of adult C. cunea to 1-dodecane is associated with the transmembrane signal transduction and dephosphorylation of some proteins. Our findings will provide useful targets for further studies on the molecular mechanism of host recognition in C. cunea.

Project description:BackgroundA large number of insect chemosensory genes from different gene subfamilies have been identified and annotated, but their functional diversity and complexity are largely unknown. A systemic examination of expression patterns in chemosensory organs could provide important information.Methodology/principal findingsWe identified 92 putative chemosensory genes by analysing the transcriptome of the antennae and female sex pheromone gland of the purple stem borer Sesamia inferens, among them 87 are novel in this species, including 24 transcripts encoding for odorant binding proteins (OBPs), 24 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 39 for odorant receptors (ORs) and 3 for ionotropic receptors (IRs). The transcriptome analyses were validated and quantified with a detailed global expression profiling by Reverse Transcription-PCR for all 92 transcripts and by Quantitative Real Time RT-PCR for selected 16 ones. Among the chemosensory gene subfamilies, CSP transcripts are most widely and evenly expressed in different tissues and stages, OBP transcripts showed a clear antenna bias and most of OR transcripts are only detected in adult antennae. Our results also revealed that some OR transcripts, such as the transcripts of SNMP2 and 2 IRs were expressed in non-chemosensory tissues, and some CSP transcripts were antenna-biased expression. Furthermore, no chemosensory transcript is specific to female sex pheromone gland and very few are found in the heads.ConclusionOur study revealed that there are a large number of chemosensory genes expressed in S. inferens, and some of them displayed unusual expression profile in non-chemosensory tissues. The identification of a large set of putative chemosensory genes of each subfamily from a single insect species, together with their different expression profiles provide further information in understanding the functions of these chemosensory genes in S. inferens as well as other insects.

Dataset Information

Transcriptome and Expression Patterns of Chemosensory Genes in Antennae of the Parasitoid Wasp Chouioia cunea.

Publications

Transcriptome and Expression Patterns of Chemosensory Genes in Antennae of the Parasitoid Wasp Chouioia cunea.

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