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Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution.

ABSTRACT: We developed a scanning hard x-ray microscope using a new class of x-ray nano-focusing optic called a multilayer Laue lens and imaged a chromosome with nanoscale spatial resolution. The combination of the hard x-ray's superior penetration power, high sensitivity to elemental composition, high spatial-resolution and quantitative analysis creates a unique tool with capabilities that other microscopy techniques cannot provide. Using this microscope, we simultaneously obtained absorption-, phase-, and fluorescence-contrast images of Pt-stained human chromosome samples. The high spatial-resolution of the microscope and its multi-modality imaging capabilities enabled us to observe the internal ultra-structures of a thick chromosome without sectioning it.

SUBMITTER: Yan H

PROVIDER: S-EPMC4742846 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution.

Yan Hanfei H Nazaretski Evgeny E Lauer Kenneth K Huang Xiaojing X Wagner Ulrich U Rau Christoph C Yusuf Mohammed M Robinson Ian I Kalbfleisch Sebastian S Li Li L Bouet Nathalie N Zhou Juan J Conley Ray R Chu Yong S YS

Scientific reports 20160205

We developed a scanning hard x-ray microscope using a new class of x-ray nano-focusing optic called a multilayer Laue lens and imaged a chromosome with nanoscale spatial resolution. The combination of the hard x-ray's superior penetration power, high sensitivity to elemental composition, high spatial-resolution and quantitative analysis creates a unique tool with capabilities that other microscopy techniques cannot provide. Using this microscope, we simultaneously obtained absorption-, phase-, a ...[more]

PMID: 26846188

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